The chloroplast ribosomal protein large subunit 1 interacts with viral polymerase and promotes virus infection

• DOI: 10.1093/plphys/kiab249
• Publication Date: 2021-05-30T11:07:09Z
• Authors: De-Jie Cheng; Xiao-Jie Xu; Zhi-Yong Yan; Carlos Kwesi Tettey; Le Fang; Guang-Ling Yang; Chao Geng; Yan-Ping Tian; Xiang-Dong Li
• Keywords: Ribosomal protein

Chloroplasts play an indispensable role in the arms race between plant viruses and hosts. Chloroplast proteins are often recruited by to support viral replication movement. However, mechanism which chloroplast regulate potyvirus infection remains largely unknown. In this study, we observed that Nicotiana benthamiana ribosomal protein large subunit 1 (NbRPL1), a protein, localized chloroplasts via its N-terminal 61 amino acids (transit peptide), interacted with tobacco vein banding mosaic virus (TVBMV) nuclear inclusion b (NIb), RNA-dependent RNA polymerase. Upon TVBMV infection, NbRPL1 was into 6K2-induced complexes chloroplasts. Silencing of expression reduced replication. competed NbBeclin1 bind NIb, NbBeclin1-mediated degradation NIb. Therefore, our results suggest interacts NIb chloroplasts, reduces degradation, enhances infection.