Imaging plant cells and organs with light-sheet and super-resolution microscopy

• DOI: 10.1093/plphys/kiab349
• Publication Date: 2021-07-21T19:11:59Z
• Authors: Miroslav Ovečka; Jiří Sojka; Michaela Tichá; George Komis; Jasim Basheer; Cintia Marchetti; Olga Šamajová; Lenka Kuběnová; Jozef Šamaj
• Keywords: Modalities

Abstract The documentation of plant growth and development requires integrative scalable approaches to investigate spatiotemporally resolve various dynamic processes at different levels body organization. present update deals with vigorous developments in mesoscopy, microscopy nanoscopy methods that have been translated imaging subcellular compartments, cells, tissues organs over the past 3 years aim report recent applications reasonable expectations from current light-sheet fluorescence (LSFM) super-resolution (SRM) modalities. Moreover, shortcomings limitations existing LSFM SRM are discussed, particularly for their ability accommodate samples regarding potential considering spherical aberrations or temporal restrictions prohibiting recording fast cellular three dimensions. For a more comprehensive description, advances living fixed sample preparation also included, supported by an overview labeling strategies successfully applied plants. These practically documented employing model Arabidopsis thaliana (L.) Heynh., but robust crop species such as Medicago sativa L. Hordeum vulgare Over few years, trend towards designing microscopic modalities has become apparent it is expected near future will be bridged achieve broader multiscale single platform.