Assembly of D1/D2 complexes of photosystem II: Binding of pigments and a network of auxiliary proteins

• DOI: 10.1093/plphys/kiac045
• Publication Date: 2022-01-29T20:09:03Z
• Authors: Jana Knoppová; Roman Sobotka; Jianfeng Yu; Martina Bečková; Jan Pilný; Joko P Trinugroho; Ladislav Csefalvay; David Bína; Peter J Nixon; Josef Komenda
• Keywords: Chlorophyll; 570; SP PCC 6803; high-light; Plant Biology & Botany; SYNECHOCYSTIS SP PCC-6803; 07 Agricultural and Veterinary Sciences; chlorophyll; CRYSTAL-STRUCTURE; ELECTRON-TRANSPORT; Research Articles; synechocystis sp pcc-6803; mutants; 580; REPAIR; photosynthesis; Science & Technology; EARLY STEPS; HIGH-LIGHT; Photosystem I Protein Complex; PHOTOSYNTHESIS; Plant Sciences; Pheophytins; Synechocystis; crystal-structure; Photosystem II Protein Complex; 06 Biological Sciences; CHLOROPHYLL; electron-transport; MUTANTS; sp pcc 6803; early steps; repair; Life Sciences & Biomedicine

Abstract Photosystem II (PSII) is the multi-subunit light-driven oxidoreductase that drives photosynthetic electron transport using electrons extracted from water. To investigate initial steps of PSII assembly, we used strains cyanobacterium Synechocystis sp. PCC 6803 arrested at early stages biogenesis and expressing affinity-tagged subunits to isolate reaction center assembly (RCII) complexes their precursor D1 D2 modules (D1mod D2mod). RCII preparations isolated either a His-tagged or FLAG-tagged PsbI subunit contained previously described RCIIa RCII* differ with respect presence Ycf39 factor high light-inducible proteins (Hlips) larger complex consisting bound monomeric PSI. All D1, D2, PsbI, PsbE, PsbF factors rubredoxin A Ycf48, but also detected PsbN, Slr1470, Slr0575 proteins, which all have plant homologs. The prohibitins/stomatins (Phbs) unknown function FtsH protease subunits. were active in light-induced primary charge separation chlorophylls (Chls), pheophytins, beta-carotenes, heme. D1mod consisted D1/PsbI/Ycf48 some Phb3, while D2mod D2/cytochrome b559 co-purifying PsbY, Phb1, FtsH2/FtsH3, CyanoP, Slr1470. As stably bound, Chl was not D2mod, formation appears be important for stable binding most Chls both pheophytins. We suggest can delivered I Ycf39/Hlips complexes.