High temperature induces male sterility via MYB66–MYB4–Casein kinase I signaling in cotton

• DOI: 10.1093/plphys/kiac213
• Publication Date: 2022-05-04T19:10:26Z
• Authors: Yanlong Li; Yaoyao Li; Qian Su; Yuanlong Wu; Rui Zhang; Yawei Li; Yizan Ma; Huanhuan Ma; Xiaoping Guo; Longfu Zhu; Ling Min; Xianlong Zhang
• Keywords: Tapetum; Gossypium; Brassinosteroid; Transcription

High temperature (HT) causes male sterility and decreases crop yields. Our previous works have demonstrated that sugar auxin signaling pathways, Gossypium hirsutum Casein kinase I (GhCKI), DNA methylation are all involved in HT-induced cotton. However, the mechanisms leading to distinct GhCKI expression patterns induced by HT between HT-tolerant HT-sensitive cotton anthers remain largely unknown. Here, we identified a promoter (ProGhCKI) region functions response found transcription factor GhMYB4 binds this act as an upstream positive regulator of GhCKI. In tapetum early-stage anthers, upregulated under overexpressed normal both led severe phenotypes, coupled with enhanced We also interacts GhMYB66 form heterodimer enhance its binding ProGhCKI. showed pattern similar but did not directly bind Furthermore, reduced siRNA-mediated CHH methylations promoter, which tetrad stage promoted formation GhMYB4/GhMYB66 heterodimer, turn elevated tapetum, sterility. Overall, shed light on GhMYB66-GhMYB4-GhCKI regulatory pathway anthers.