Knockout of MITOGEN-ACTIVATED PROTEIN KINASE 3 causes barley root resistance against Fusarium graminearum

• DOI: 10.1093/plphys/kiac389
• Publication Date: 2022-08-22T14:12:28Z
• Authors: Jasim Basheer; Pavol Vadovič; Olga Šamajová; Pavol Melicher; George Komis; Pavel Křenek; Michaela Králová; Tibor Pechan; Miroslav Ovečka; Tomáš Takáč; Jozef Šamaj
• Keywords: Proteomics; 2. Zero hunger; 0303 health sciences; 03 medical and health sciences; Mitogen-Activated Protein Kinase 3; Fusarium; Transcription Activator-Like Effector Nucleases; Hordeum; Reactive Oxygen Species; Research Articles; Plant Diseases

The roles of mitogen-activated protein kinases (MAPKs) in plant-fungal pathogenic interactions are poorly understood crops. Here, microscopic, phenotypic, proteomic, and biochemical analyses revealed that roots independent transcription activator-like effector nuclease (TALEN)-based knockout lines barley (Hordeum vulgare L.) MAPK 3 (HvMPK3 KO) were resistant against Fusarium graminearum infection. When co-cultured with the HvMPK3 KO lines, F. hyphae excluded to extracellular space, growth pattern was considerably deregulated, mycelia development less efficient, number appressoria-like structures their penetration potential substantially reduced. Intracellular preceded by massive production reactive oxygen species (ROS) attacked cells wild-type (WT), but ROS mitigated lines. Suppression these coincided elevated abundance catalase (CAT) ascorbate peroxidase (APX). Moreover, differential proteomic analysis downregulation several defense-related proteins WT, upregulation pathogenesis-related 1 (PR-1) cysteine proteases Proteins involved suberin formation, such as peroxidases, lipid transfer (LTPs), GDSL esterase/lipase (containing "GDSL" aminosequence motif) differentially regulated after inoculation. Consistent analysis, microscopic observations showed enhanced accumulation most likely contributing arrested infection graminearum. These results suggest TALEN-based leads root resistance rot.