Acetylome reprograming participates in the establishment of fruit metabolism during polyploidization in citrus

• DOI: 10.1093/plphys/kiac442
• Publication Date: 2022-09-22T13:39:25Z
• Authors: Miao Zhang; Feng-Quan Tan; Yan-Jie Fan; Ting-Ting Wang; Xin Song; Kai-Dong Xie; Xiao-Meng Wu; Fan Zhang; Xiu-Xin Deng; Jude W Grosser; Wen-Wu Guo
• Keywords: Reprogramming; Secondary metabolism; Dominance (genetics)

Abstract Polyploidization leads to novel phenotypes and is a major force in evolution. However, the relationship between evolution of new traits variations post-translational modifications (PTM) proteins during polyploidization has not been studied. Acetylation lysine residues common protein PTM that plays critical regulatory role central metabolism. To test whether changes metabolism citrus fruit associated with reprogramming acetylation (Kac) non-histone allotetraploidization, we performed global acetylome analysis fruits from synthetic allotetraploid its diploid parents. A total 4,175 Kac sites were identified on 1,640 involved wide range traits. In allotetraploid, parental dominance (i.e. resemblance one two parents) specific traits, such as acidity flavonol metabolism, was highly level pertinent enzymes. This association due Kac-mediated regulation enzyme activity. Moreover, probably contributes discordance transcriptomic proteomic allotetraploidization. The can be partially explained by expression pattern several deacetylases (KDACs). Overexpression silent information regulator 2 (CgSRT2) histone deacetylase 8 (CgHDA8) diverted metabolic flux primary secondary restored status which expressed attenuated levels CgSRT2 CgHDA8. Additionally, KDAC inhibitor treatment greatly altered fruit. Collectively, these findings reveal important trait polyploidization.