Ice plant root plasma membrane aquaporins are regulated by clathrin-coated vesicles in response to salt stress

• DOI: 10.1093/plphys/kiac515
• Publication Date: 2022-11-16T15:42:52Z
• Authors: María Fernanda Gó...; Julio César Amezc...; Paul Rosas-Santiago; Eric Edmundo Hern...; Luis Alberto de L...; Jorge Luis Ruiz-S...; Rosario Vera-Estr...; Omar Pantoja
• Keywords: Mesembryanthemum crystallinum

Abstract The regulation of root Plasma membrane (PM) Intrinsic Protein (PIP)-type aquaporins (AQPs) is potentially important for salinity tolerance. However, the molecular and cellular details underlying this process in halophytes remain unclear. Using free-flow electrophoresis label-free proteomics, we report that increased abundance PIPs at PM halophyte ice plant (Mesembryanthemum crystallinum L.) roots under conditions regulated by clathrin-coated vesicles (CCV). To understand regulation, analyzed several components M. CCV complexes: clathrin light chain (McCLC) subunits μ1 μ2 adaptor protein (AP) complex (McAP1μ McAP2μ). Co-localization analyses revealed association between McPIP1;4 McAP2μ McPIP2;1 McAP1μ, observations corroborated mbSUS assays, suggesting AQP control CCV. ability to form homo- hetero-oligomers was tested confirmed, as well their activity water channels. Also, found phosphorylation only response salt stress. Our results indicate from might be through trafficking phosphorylation, impacting localization, transport activity, conditions.