microRNA408 and its encoded peptide regulate sulfur assimilation and arsenic stress response in Arabidopsis

• DOI: 10.1093/plphys/kiad033
• Publication Date: 2023-01-22T21:31:11Z
• Authors: Ravi Shankar Kumar; Hiteshwari Sinha; Tapasya Datta; Mehar Hasan Asif; Prabodh Kumar Tri...
• Keywords: Assimilation (phonology)

MicroRNAs (miRNAs) are small non-coding RNAs that play a central role in regulating various developmental and biological processes. The expression of miRNAs is differentially modulated response to biotic abiotic stresses. Recent findings have shown some pri-miRNAs encode regulatory peptides known as microRNA-encoded (miPEPs). miPEPs regulate the growth development plants by modulating corresponding miRNA expression; however, these under different stress conditions remains unexplored. Here, we report pri-miR408 encodes peptide, miPEP408, regulates miR408, its targets, associated phenotype Arabidopsis. We also apart from Plantacyanin (ARPN) Laccase3 (LAC3), targets glutathione S-transferase (GSTU25) plays sulfur assimilation exhibits range detoxification activities with environmental pollutant. Plants overexpressing miR408 showed severe sensitivity low (LS), arsenite As(III), LS + As(III) stress, while mutants developed using CRISPR/Cas9 approach tolerance. Transgenic lines phenotypic alteration modulation genes involved reduction pathway affect sulfate accumulation. Similar lines, exogenous application synthetic miPEP408 miPEP408OX led LS, combined compared control. This study suggests involvement heavy metal nutrient deficiency responses through pathway.