Changes in cell wall composition due to a pectin biosynthesis enzyme GAUT10 impact root growth

• DOI: 10.1093/plphys/kiad465
• Publication Date: 2023-08-22T11:46:56Z
• Authors: Linkan Dash; Sivakumar Swamina...; Jan Šimura; Caitlin Leigh P G...; Christian Montes; Neel Solanki; Ludvin Mejia; Karin Ljung; Olga A Zabotina; Dior R Kelley
• Keywords: Pectin; Root hair; Lateral root

Arabidopsis (Arabidopsis thaliana) root development is regulated by multiple dynamic growth cues that require central metabolism pathways such as β-oxidation and auxin. Loss of the pectin biosynthesizing enzyme GALACTURONOSYLTRANSFERASE 10 (GAUT10) leads to a short-root phenotype under sucrose-limited conditions. The present study focused on determining specific contributions GAUT10 composition in primary roots underlying defects associated with gaut10 roots. Using live-cell microscopy, we determined reduced due reduction both apical meristem size epidermal cell elongation. In addition, was required for normal hemicellulose Specifically, loss led galacturonic acid xylose walls altered presence rhamnogalacturonan-I (RG-I) homogalacturonan (HG) polymers root. Transcriptomic analysis compared wild type uncovered hundreds genes differentially expressed mutant, including related auxin peroxisome function. Consistent these results, signaling were modified sucrose-dependent linked based hypersensitivity indole-3-butyric (IBA) an epistatic interaction TRANSPORTER OF IBA1 (TOB1). Altogether, data support growing body evidence suggesting may influence activity.