Promoting γ-aminobutyric acid accumulation to enhances saline-alkali tolerance in tomato

• DOI: 10.1093/plphys/kiae446
• Publication Date: 2024-08-26T17:56:01Z
• Authors: Jingrong Wang; Yong Zhang; Junzheng Wang; Fang Ma; Linyang Wang; Xiangqiang Zhan; Guobin Li; Songshen Hu; Abid Khan; Haoran Dang; Tianlai Li; Xiaohui Hu
• Keywords: Aminobutyric acid

Abstract Saline-alkali stress is a widely distributed abiotic that severely limits plant growth. γ-Aminobutyric acid (GABA) accumulates rapidly in plants under saline-alkali stress, but the underlying molecular mechanisms and associated regulatory networks remain unclear. Here, we report MYB-like protein, I-box binding factor (SlMYBI), which positively regulates tolerance through induced GABA accumulation by directly modulating glutamate decarboxylase (GAD) gene SlGAD1 tomato (Solanum lycopersicum L.). Overexpression of increased levels decreased reactive oxygen species while silencing further suggested plays an active role synthesis tomato. In addition, found SlMYBI activates transcription. Both overexpression editing using CRISPR–Cas9 showed expression. Furthermore, interaction SlNF-YC1 with enhanced transcriptional activity on to improve Interestingly, ethylene signaling was involved response RNA-seq analysis SlGAD1-overexpressing lines. This study elucidates involvement regulation. Specifically, SlMYBI–SlNF-YC1 module stress.