The Tetratricopeptide repeat protein TaTPR-B1 regulates spike compactness in bread wheat

• DOI: 10.1093/plphys/kiae546
• Publication Date: 2024-10-15T18:46:13Z
• Authors: Jun Zhu; Feng Huang; Huijie Zhai; Yi Zheng; Jiazheng Yu; Zhaoyan Chen; Yujie Fan; Huanhuan Zhao; Qixin Sun; Rongqi Liang; Zhongfu Ni
• Keywords: Indel; Common wheat; Doubled haploidy; Inbred strain

Abstract Spike compactness (SC) is strongly associated with wheat (Triticum aestivum L.) grain yield. In this study, we conducted a quantitative trait locus (QTL) analysis using doubled haploid (DH) population derived from cross between two common varieties contrasting spike morphology, revealing 16 stable QTLs SC. The effect of major QTL, QSc.cau-6B.1, was validated in 231 F7 recombinant inbred lines (RILs) the same as DH population. Using residual heterozygous (RHLs), delimited QSc.cau-6B.1 to an approximately 0.5-Mbp physical interval containing four high-confidence genes. tetratricopeptide repeat-TraesCS6B03G1214400 (TaTPR-B1) priority candidate gene according sequence and expression variations near-isogenic lines. Accordingly, TaTPR-B1 knockout variety ‘CB037’ significantly increased SC compared wild type (WT). Conversely, overexpression ‘Fielder’ decreased WT. Moreover, developed PCR-based marker targeting 32-bp insertion/deletion (InDel) alleles, which could be practical valuable modern breeding programs for diagnostic purposes. Collectively, these findings provide insight into genetic basis present target breeder-friendly pyramid breeding.