Skin organoid transplantation promotes tissue repair with scarless in frostbite

• DOI: 10.1093/procel/pwae055
• Publication Date: 2024-10-04T08:02:44Z
• Authors: Wenwen Wang; Pu Liu; Wendi Zhu; Tianwei Li; Ying Wang; Yujie Wang; Jun Li; Jie Ma; Ling Leng
• Keywords: Frostbite; Skin repair; Myofibroblast

Frostbite is the most common cold injury and caused by both immediate cold-induced cell death gradual development of localized inflammation tissue ischemia. Delayed healing frostbite often leads to scar formation, which not only causes psychological distress but also tends result in secondary malignant tumors. Therefore, a rapid method for wounds urgently needed. Herein, we used mouse skin model evaluate recovery process after frostbite. Moreover, single-cell transcriptomics was determine patterns changes monocytes, macrophages, epidermal cells fibroblasts during Most importantly, human-induced pluripotent stem (hiPSC) -derived organoids combining with gelatin-hydrogel were constructed treatment The results showed that organoid significantly accelerated wound reducing early increasing proportions cells. later stage healing, reduced overall fibroblasts, fibroblast-to-myofibroblast transition regulating integrin α5β1-FAK pathway, remodeled extracellular matrix (ECM) through degradation reassembly mechanisms, facilitating restoration physiological ECM abundance associated abnormal formation. These highlight potential application promoting reversal frostbite-related functions. This study provides new therapeutic alternative patients suffering from disfigurement dysfunction