Chromatin landscape alteration uncovers multiple transcriptional circuits during memory CD8+ T-cell differentiation

• DOI: 10.1093/procel/pwaf003
• Publication Date: 2025-01-13T13:49:41Z
• Authors: Qiao Liu; Wei Dong; Rong Liu; Luming Xu; Ling Ran; Ziying Xie; Shun Lei; Xingxing Su; Zhengliang Yue; Dan Xiong; Lisha Wang; Shuqiong Wen; Yan Zhang; Jianjun Hu; Chenxi Qin; Yongchang Chen; Bo Zhu; Xiangyu Chen; Xia Wu; Lifan Xu; Qizhao Huang; Yingjiao Cao; Lilin Ye; Zhonghui Tang
• Keywords: Reprogramming

Abstract Extensive epigenetic reprogramming involves in memory CD8+ T-cell differentiation. The elaborate rewiring underlying the heterogeneous functional states of T cells remains hidden. Here, we profile single-cell chromatin accessibility and map enhancer-promoter interactomes to characterize differentiation trajectory cells. We reveal that under distinct regulations, early activated divergently originated for short-lived effector precursor also uncover a defined leading conversion from central during formation. Additionally, illustrate regulatory mechanisms long-lasting versus transient transcription regulation Finally, confirm essential roles Sox4 Nrf2 developing cells, respectively, validate cell state-specific enhancers regulating Il7r using CRISPR-Cas9. Our data pave way understanding mechanism formation