Effects of Feed Additives and Mixed Eimeria Species Infection on Intestinal Microbial Ecology of Broilers

• DOI: 10.1093/ps/85.12.2106
• Publication Date: 2014-02-19T00:23:51Z
• Authors: M.E. Hume; S. Clemente-Herná...; E.O. Oviedo-Rondón
• Keywords: Monensin; Bacitracin; Eimeria maxima; Feed additive

Evaluation of digestive microbial ecology is necessary to understand effects growth-promoting feed. In the current study, dynamics intestinal communities (MC) were examined in broilers fed diets supplemented with a combination antibiotic (bacitracin methylene disalicylate) and ionophore (Coban 60), containing 1 2 essential oil (EO) blends, Crina Poultry (CP) Alternate (CA). Five treatments analyzed: 1) unmedicated uninfected control; 2) infected 3) feed additives monensin + 60; AI); 4) EO blend CP; 5) CA. Additives mixed into basal mixture, adjusted 100 ppm. Chicks by oral gavage at 19 d age Eimeria acervulina, maxima, tenella. Duodenal, ileal, cecal samples taken from 12 birds per treatment just before 7 after challenge; each pooled give final number 6 total; all frozen until used for DNA extraction. Denaturing gradient gel electrophoresis was examine PCR-amplified fragments bacterial 16S ribosomal variable region. Results are presented as percentages similarity coefficients (SC). Dendrograms PCR amplicon or band patterns indicated MC differences due location, additives, cocci challenge. Essential blends CP CA affected gut sections. Each had different over MC, they differed most instances AI group. The challenge caused drastic population shifts duodenal, sections (36.7, 55.4, 36.2% SC, respectively). Diets supported higher SC between pre- postchallenge (89.9, 83.3, 76.4%) than (81.8., 57.4, 60.0%). We concluded that coccidia MC. These modulated better AI, avoiding