Insulin secretory actions of polyphenols of Momordica charantia regulate glucose homeostasis in alloxan-induced type 2 diabetic rats

• DOI: 10.1093/rpsppr/rqae005
• Publication Date: 2024-02-28T22:22:15Z
• Authors: Prawej Ansari; Joyeeta T Khan; Mousume Soultana; Lauren Hunter; Suraiya Chowdhury; Suriya K Priyanka; Saikat R Paul; Peter R Flatt; Yasser H A Abdel-...
• Keywords: Alloxan; Homeostasis

Abstract Objective Momordica charantia, commonly known as bitter gourd, is traditionally used remedies for various diseases including diabetes. The main objective of this study to investigate the in vitro and vivo insulinotropic antidiabetic effects an 80% ethanolic extract M. charantia (EEMC) fruit, well underlying molecular mechanism involved preliminary phytochemical screening. Methods insulin secretion was measured using clonal pancreatic BRIN-BD11 β-cells isolated mouse islets. ability EEMC inhibit carbohydrate digestive enzymes glucose absorption scavenge free radicals assessed via starch digestion, diffusion, 2,2-diphenyl-1-picrylhydrazyl assay methods. on a variety metabolic parameters were evaluated alloxan-induced type 2 diabetic rats, lipid profile. Finally, screening conducted identify active phytoconstituents. Key findings increased release through KATP-dependent/cyclic adenosine monophosphate pathway, which depolarizes β-cell membrane elevates intracellular calcium. It also inhibited radicals, suggesting its potential delay gastric emptying, attenuate oxidative stress, reduce inflammatory cytokines. In studies showed that improves oral tolerance, food intake, fasting blood glucose, plasma insulin, lipids promotes intestinal motility. phytoconstituents EEMC, such flavonoids, alkaloids, tannins, saponins, steroids, glycosides, are likely responsible these effects. Conclusion antihyperglycemic properties indicate it might be promising candidate diabetes management. However, additional into application essential.