Vibration Enhances Interleukin-8 Release in a Cell Model of Snoring-Induced Airway Inflammation

• DOI: 10.1093/sleep/28.10.1312
• Publication Date: 2017-02-05T11:24:02Z
• Authors: Ferranda Puig; Felix Rico; Isaac Almendros; Josep M. Montserrat; Daniel Navajas; Ramon Farre
• Keywords: Stimulus (psychology)

To test a cell model of snoring-induced airway inflammation and to assess whether vibration stimulus simulating the one experienced by tissues in snoring patients induces epithelial cells. Prospective controlled study culture. University laboratory. Human bronchial cells (BEAS-2B line). Cell cultures were subjected (60 Hz, ± 0.3 mm) for time periods 6 hours, 12 24 hours. The vibratory was applied with without treatment inhibitors 3 main pathways mitogen-activated protein kinases (MAPK): p38, MEK1/2, JNK. effect assessed comparing proliferation release interleukin-8 (IL-8; measured enzyme-linked immunosorbent assay) (both when treated untreated MAPK inhibitors) controls. Application up hours did not significantly modify proliferation. By contrast, concentration IL-8 supernatant increased after vibration. inhibition JNK reduced overexpression resulting from stimulus. A mechanical triggered an inflammatory cascade, as reflected increase mediated pathways. novel developed is potentially applicable studying effects due different types (epithelial, endothelial, muscular, neuronal) involved pathophysiology during respiratory sleep disturbances.