Accelerated Species Inventory on Madagascar Using Coalescent-Based Models of Species Delineation

• DOI: 10.1093/sysbio/syp027
• Publication Date: 2009-07-02T02:34:15Z
• Authors: Michael T. Monaghan; Ruth Wild; Miranda Elliot; Tomochika Fujisawa; Michael Balke; Daegan J.G. Inward; David C. Lees; Ravo Ranaivosolo; Paul Eggleton; Timothy G. Barrac...; Alfried P. Vogler
• Keywords: Coalescent theory; DNA Barcoding; Species complex; Endemism

High-throughput DNA sequencing has the potential to accelerate species discovery if it is able recognize evolutionary entities from sequence data that are comparable species. The general mixed Yule-coalescent (GMYC) model estimates boundary surveys by identifying independently evolving lineages as a transition coalescent speciation branching patterns on phylogenetic tree. Applied here 12 families 4 orders of insects in Madagascar, we used delineate 370 putative mitochondrial variation among 1614 individuals. These were compared with nuclear genome and morphological identification found be highly congruent (98% 94%). We developed modified GMYC allows for variable lineages. This revised increased congruence morphology (97%), suggesting threshold better reflects clustering into biological Local endemism was pronounced all 5 insect groups. Most (60–91%) haplotypes (88–99%) at only 1 study sites (40–1000 km apart). resulted 37% increase numbers using diagnostic nucleotides population aggregation analysis. Sample sizes between 7 10 individuals represented above which there minimal genetic diversity, broadly agreeing theory other empirical studies. Our results > 1.4 Mb suggest captures boundaries those traditional methods without need prior hypotheses coherence. provides method biodiversity assessment single-locus or environmental samples while building globally available taxonomic database future identifications.