Triazole fungicides induce adipogenesis and repress osteoblastogenesis in zebrafish

• DOI: 10.1093/toxsci/kfad031
• Publication Date: 2023-03-23T00:19:27Z
• Authors: Savini Thrikawala; Fahmi Mesmar; Beas Bhattacharya; Maram Muhsen; Srijita Mukhopadhyay; Sara Flores; Sanat Upadhyay; Leoncio Vergara; Jan-Åke Gustafsson; Cecilia Williams; Maria Bondesson
• Keywords: Danio; Triazole

Abstract Triazoles are a major group of azole fungicides commonly used in agriculture, and veterinary human medicine. Maternal exposure to certain triazole antifungal medication causes congenital malformations, including skeletal malformations. We hypothesized that triazoles as pesticides agriculture also pose risk causing malformations developing embryos. In this study, teratogenic effects three triazoles, cyproconazole, paclobutrazol, triadimenol, were investigated zebrafish, Danio rerio. Exposure the caused bone cartilage zebrafish larvae. Data from whole-embryo transcriptomics with cyproconazole suggested compound induces adipogenesis while repressing development. Confirming finding, expression selected marker genes significantly downregulated determined by quantitative PCR. The adipogenic was upregulated triazoles. Furthermore, each induced lipid droplet formation vitro 3T3-L1 pre-adipocyte cells. vivo larvae, accumulation. These results suggest promotes at expense development, thus they expand chemical bona fide fat switchers.