Integrative Analysis of miRNA and Inflammatory Gene Expression After Acute Particulate Matter Exposure

• DOI: 10.1093/toxsci/kft013
• Publication Date: 2013-01-29T02:04:21Z
• Authors: Valeria Motta; Laura Angelici; Francesco Nordio; Valentina Bollati; Serena Fossati; Fabio Frascati; Valentina Tinaglia; Pier Alberto Bert...; Cristina Battaglia; Andrea A. Baccarelli
• Keywords: Fold change; Biological pathway; Candidate gene

MicroRNAs (miRNAs) are environmentally sensitive inhibitors of gene expression that may mediate the effects metal-rich particulate matter (PM) and toxic metals on human individuals. Previous environmental miRNA studies have investigated a limited number candidate miRNAs not yet evaluated functional expression. In this study, we wanted to identify PM-sensitive using microarray profiling matched baseline postexposure RNA from foundry workers with well-characterized exposure PM characterize relations inflammatory genes. We applied analysis 847 real-time PCR 18 genes blood samples collected at after 3 days work (postexposure). identified differentially expressed (fold change [FC] > 2 p < 0.05) correlated their associated performed in silico network MetaCore v6.9 biological pathways connecting miRNA-mRNA pairs. Microarray four were compared samples, including miR-421 (FC = 2.81, 0.001), miR-146a 2.62, 0.007), miR-29a 2.91, let-7g 2.73, 0.019). Using false discovery date adjustment for multiple comparisons, found 11 pairs involving 4 database interactions all pairs, which ranged direct mRNA targeting complex intermediates. Acute affect regulation through PM-responsive directly or indirectly control