Determination of Toxic Activities inBothropsspp. Snake Venoms Using Animal-Free Approaches: Correlation BetweenIn VitroVersusIn VivoAssays

• DOI: 10.1093/toxsci/kfv140
• Publication Date: 2015-07-10T01:23:54Z
• Authors: Letícia Lopes de ...; Stephanie Stransky; Clara Guerra-Duarte; Ana Flor-Sá; Francisco Santos ...; Evanguedes Kalapo...; Carlos Chávez-Oló...
• Keywords: Bothrops; Bothrops jararaca; Myotoxin; In vitro toxicology; Hyaluronidase; Antivenom

The main purpose of this study is to investigate the in vitro toxic effects 5 Bothrops spp. snake venoms, which are part antigenic mixture used for production Brazilian antivenom, and evaluate their correlation with vivo activities venoms. analysis could be helpful replacement living animals experimentation bioassay. Cytotoxicity, L-amino acid oxidase (LAAO), proteolitic (serine metalloproteinase), hyaluronidase (Hyal), phospholipase A2 (PLA2) were estimated coefficient was determined each activity relation lethality, edema, hemorrhage necrosis induced live by B. jararaca, alternatus, jararacussu, neuwiedi, moojeni lethal mice highly related Hyal (r = 0.94, p < .05), edema PLA2 whereas necrotizing showed high LAAO 0.83, .05). A very significant between cytotoxicity also observed 0.97,