From the Cover: Metabolism Modulation in Different Organs by Silver Nanoparticles: An NMR Metabolomics Study of a Mouse Model

• DOI: 10.1093/toxsci/kfx142
• Publication Date: 2017-07-11T12:45:28Z
• Authors: Ivana Jarak; Joana Carrola; António S. Barros; Ana M. Gil; Maria de Lourdes ...; Maria Luisa Corvo; Iola F. Duarte
• Keywords: Glycogenesis; Glycogenolysis; Metabolic pathway; Detoxification

Although silver nanoparticles (AgNPs) are widely disseminated and show great potential in the biomedical field, there is a recognized need to better understand their action at metabolic functional levels. In this work, we have used NMR metabolomics, together with conventional clinical chemistry histological examination, characterize multi-organ systemic responses AgNPs intravenously administered mice 8 mg/kg body weight (a dose not eliciting overt toxicity). The major target organs of accumulation, liver spleen, showed greatest changes, clear 2-stage response. particular, dosed was found switch from glycogenolysis lipid storage, 6 h postinjection, glycogenesis lipolysis, subsequent times up 48 h. Moreover, metabolites related antioxidative defense, immunoregulation detoxification seemed play crucial role avoiding hepatic damage. spleen several early including depletion amino acids, possibly reflecting impairment hemoglobin recycling, while only few differences remained postinjection. heart, shift towards TCA cycle intensification increased ATP production reflected beneficial adaptation presence AgNPs. On other hand, appeared be down regulated lungs injected mice, which signs inflammation. Thekidneys mildest response Overall, study has shown that metabolomics powerful tool monitor invivo nanoparticles, revealing unforeseen effects.