Taxonomically Different Co-Microsymbionts of a Relict Legume, Oxytropis popoviana, Have Complementary Sets of Symbiotic Genes and Together Increase the Efficiency of Plant Nodulation

• DOI: 10.1094/mpmi-01-18-0011-r
• Publication Date: 2018-03-02T14:02:50Z
• Authors: Vera I. Safronova; Andrey A. Belimov; Anna L. Sazanova; Elizaveta R. Chirak; Alla V. Verkhozina; Irina G. Kuznetsova; Evgeny E. Andronov; Jan V. Puhalsky; Igor A. Tikhonovich
• Keywords: Bradyrhizobium; Housekeeping gene; Bradyrhizobium japonicum; Strain (injury)

Ten rhizobial strains were isolated from root nodules of a relict legume Oxytropis popoviana Peschkova. For identification the isolates, sequencing rrs, internal transcribed spacer region, and housekeeping genes recA, glnII, rpoB was used. Nine fast-growing isolates Mesorhizobium-related; eight identified as M. japonicum one isolate belonged to kowhaii. The only slow-growing Bradyrhizobium sp. Two strains, Opo-242 strain Opo-243, same nodule. Symbiotic these searched throughout whole-genome sequences. common nodABC other symbiotic required for plant nodulation nitrogen fixation present in Opo-242. Strain Opo-243 did not contain principal nod, nif, fix genes; however, five (nodP, nodQ, nifL, nolK, noeL) affecting specificity plant-rhizobia interactions but absent detected. could induce significantly accelerated nodule formation after coinoculation with Thus, we demonstrated that taxonomically different archaic system can be co-microsymbionts infecting promoting process due complementary sets genes.