Rapid Methodologies for AssessingPseudomonas syringaepv.actinidiaeColonization and Effector-Mediated Hypersensitive Response in Kiwifruit

• DOI: 10.1094/mpmi-02-21-0043-r
• Publication Date: 2021-04-09T10:52:45Z
• Authors: Jay Jayaraman; Abhishek Chatterjee; Shannon Hunter; Ronan Chen; Erin A. Stroud; Hassan Saei; Stephen Hoyte; Simon Deroles; Jibran Tahir; Matthew D. Templeton; Cyril Brendolise
• Keywords: Pseudomonas syringae; Pseudomonas fluorescens

The infection of Pseudomonas syringae pv. actinidiae in kiwifruit is currently assessed by numerous methodologies, each with their own limitations. Most studies are based on either a laborious method growth quantification the pathogen or qualitative assessments visual scoring following stem cutting inoculation. Additionally, when assessing for resistance against specific effectors, confounding interactions between multiple genes can make mapping phenotypes nearly impossible. Here, we present robust alternative methods to quantify load rapid bacterial DNA PCR, use fluorescens, and transient reporter eclipse assay conferred isolated avirulence genes. These assays compare well plate counts assess colonization as result plant activation. DNA-based quantification, coupled P. fluorescens independently identify genes, rapid, highly reproducible, scalable high-throughput screens cultivars genotypes. Application these methodologies will allow identification resistant they recognize, facilitating gene discovery breeding programs. [Formula: see text] Copyright © 2021 Author(s). This an open access article distributed under CC BY-NC-ND 4.0 International license .