Rhizobial Microsymbionts of Kamchatka Oxytropis Species Possess Genes of the Type III and VI Secretion Systems, Which Can Affect the Development of Symbiosis

• DOI: 10.1094/mpmi-05-20-0114-r
• Publication Date: 2020-07-20T14:17:59Z
• Authors: Vera I. Safronova; Polina V. Guro; Anna L. Sazanova; Irina G. Kuznetsova; Andrey A. Belimov; Valentin V. Yakubov; Elizaveta R. Chirak; Alexey М. Afonin; Yuri V. Gogolev; Evgeny E. Andronov; Igor A. Tikhonovich
• Keywords: Mesorhizobium; Sinorhizobium; Nod factor

A collection of rhizobial strains isolated from root nodules the narrowly endemic legume species Oxytropis erecta, O. anadyrensis, kamtschatica, and pumilio originating Kamchatka Peninsula (Russian Federation) was obtained. Analysis 16S ribosomal RNA gene sequence showed a significant diversity isolates belonging to families Rhizobiaceae (genus Rhizobium), Phyllobacteriaceae (genera Mesorhizobium, Phyllobacterium), Bradyrhizobiaceae Bosea, Tardiphaga). plant nodulation assay that only genus Mesorhizobium could form nitrogen-fixing on plants. The M. loti 582 huakuii 583, in addition symbiotic clusters, possessed genes type III VI secretion systems (T3SS T6SS, respectively), which can influence host specificity strains. These formed two types (elongated rounded) kamtschatica roots. We suggest this phenomenon may result Nod factor-dependent -independent strategies. obtained are interest for further study T3SS T6SS function their role development rhizobium-legume symbiosis. prospects using rhizobia having both related nonsymbiotic strategies enhance efficiency plant-microbe interactions by expanding increasing discussed.