Berberine Bridge Enzyme-like Oligosaccharide Oxidases Act as Enzymatic Transducers Between Microbial Glycoside Hydrolases and Plant Peroxidases

• DOI: 10.1094/mpmi-05-22-0113-ta
• Publication Date: 2022-06-15T18:36:06Z
• Authors: Anna Scortica; Moira Giovannoni; Valentina Scafati; Francesco Angelucci; Felice Cervone; Giulia De Lorenzo; Manuel Benedetti; Benedetta Mattei
• Keywords: 0301 basic medicine; Glycoside Hydrolases; Transducers; lignin; Oligosaccharides; auxin, berberine bridge enzyme-like protein, DAMPs, glycoside hydrolase, H2O2, lignin, oligosaccharide oxidase, peroxidase, plant immunity; Microbiology; DAMPs; H(2)O(2); auxin; berberine bridge enzyme-like protein; glycoside hydrolase; lignin; oligosaccharide oxidase; peroxidase; plant immunity; berberine bridge enzyme-like protein; 03 medical and health sciences; Cellulase; glycoside hydrolase; DAMPs; H(2)O(2); auxin; berberine bridge enzyme-like protein; glycoside hydrolase; lignin; oligosaccharide oxidase; peroxidase; plant immunity; Glycoside Hydrolases; Hydrogen Peroxide; Indoleacetic Acids; Oligosaccharides; Oxidoreductases, N-Demethylating; Peroxidases; Plants; Polygalacturonase; Transducers; Cellulase; Oxidoreductases; DAMPs; 0303 health sciences; Indoleacetic Acids; H(2)O(2); Botany; Oxidoreductases, N-Demethylating; Hydrogen Peroxide; Plants; QR1-502; Polygalacturonase; Peroxidases; QK1-989; auxin; Oxidoreductases

Oligogalacturonide (OG)-oxidase 1 (OGOX1) and cellodextrin (CD)-oxidase (CELLOX) are plant berberine bridge enzyme-like oligosaccharide oxidases that oxidize OGs and CDs, cell-wall fragments with the nature of damage-associated molecular patterns. The oxidation of OGs and CDs attenuates their elicitor activity and concomitantly releases H2O2. By using a multiple enzyme–based assay, we demonstrate that the H2O2 generated downstream of the combined action between a fungal polygalacturonase and OGOX1 or an endoglucanase and CELLOX can be directed by plant peroxidases (PODs) either towards a reaction possibly involved in plant defense, such as the oxidation of monolignol or a reaction possibly involved in a developmental event, such as the oxidation of auxin (indole-3-acetic acid), pointing to OGOX1 and CELLOX as enzymatic transducers between microbial glycoside hydrolases and plant PODs. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .