Alterations in Sucrose and Phenylpropanoid Metabolism Affected by BABA-Primed Defense in Postharvest Grapes and the Associated Transcriptional Mechanism

• DOI: 10.1094/mpmi-06-21-0142-r
• Publication Date: 2021-08-19T19:23:30Z
• Authors: Chunhong Li; Kaituo Wang; Changyi Lei; Shifeng Cao; Yixiao Huang; Nana Ji; Feng Xu; Yonghua Zheng
• Keywords: Phenylpropanoid; WRKY protein domain; Phytoalexin

Defense elicitors can induce fruit disease resistance to control postharvest decay but may incur quality impairment. Our present work aimed investigate the against Botrytis cinerea induced by elicitor β-aminobutyric acid (BABA) and elucidate specific transcriptional mechanism implicated in defense-related metabolic regulations. The functional dissection results demonstrated that, after inoculation with fungal necrotroph B. cinerea, a suite of critical genes encoding enzymes related sucrose metabolism phenylpropanoid pathway priming defense grapes were transcriptionally treatment 10 mM BABA. In contrast, more UDP-glucose, shared precursor metabolism, be redirected for synthesis phytoalexins, including trans-resveratrol ɛ-viniferin, 100 BABA-treated grapes, resulting direct compromised soluble sugar contents. An R2R3-type MYB protein from Vitis vinifera, VvMYB44, was isolated characterized. VvMYB44 expression significantly upon expressed defensive reaction. Subcellular localization, yeast two-hybrid, coimmunoprecipitation assays revealed that nuclear-localized physically interacted salicylic acid-responsive transcription coactivator NPR1 vivo expression. addition, directly bound promoter regions metabolism-related transactivated their expression, thus tipping balance antifungal compound accumulation maintenance. Hence, these suggest 2R-type might potential positive participant BABA-induced grape berries contributes avoiding excessive consumption sugars during storage.[Formula: see text] Copyright © 2021 Author(s). This is an open access article distributed under CC BY-NC-ND 4.0 International license.