Methylome Response to Proteasome Inhibition by Pseudomonas syringae Virulence Factor Syringolin A

• DOI: 10.1094/mpmi-06-23-0080-r
• Publication Date: 2023-07-07T00:15:56Z
• Authors: Diane Marie Valer...; Louis Tirot; Stefan Grob; Pauline Emilie Ju...
• Keywords: Pseudomonas syringae; Virulence factor

DNA methylation is an important epigenetic mark required for proper gene expression and silencing of transposable elements. patterns can be modified by environmental factors such as pathogen infection, in which modification associated with plant resistance. To counter the defense pathways, pathogens produce effector molecules, several act proteasome inhibitors. Here, we investigated effect inhibition bacterial virulence factor syringolin A (SylA) on genome-wide methylation. We show that SylA treatment results increase at centromeric pericentromeric regions Arabidopsis chromosomes. identify CHH differentially methylated (DMRs) are enriched proximity transcriptional start sites. does not result significant changes small RNA composition. However, genome activity observed, including a strong upregulation resistance genes located chromosomal arms. hypothesize could linked to some atypical members de novo pathway, namely AGO3, AGO9, DRM1. Our data suggests resulting from effectors part epi-genomic arms race against pathogens. [Formula: see text] Copyright © 2023 The Author(s). This open access article distributed under CC BY-NC-ND 4.0 International license.