The Cotton miR477-CBP60A Module Participates in Plant Defense Against Verticillium dahlia

• DOI: 10.1094/mpmi-10-19-0302-r
• Publication Date: 2019-12-23T14:19:51Z
• Authors: Guang Hu; Mengyan Hao; Le Wang; Jianfen Liu; Zhennan Zhang; Ye Tang; Qingzhong Peng; Zuoren Yang; Jiahe Wu
• Keywords: Verticillium dahliae; Verticillium; Verticillium wilt

Previous reports have shown that, when Verticillium dahliae localizes at the root surface, many microRNAs (miRNAs) were identified early induction stage. Here, we constructed two groups from timepoints of small RNA (sRNA) in cotton responses to V. later stage, pathogen localizing interior tissue. We 71 known and 378 novel miRNAs six libraries pathogen-induced control sRNAs. Combined with degradome sRNA sequencing, 178 corresponding miRNA target genes identified, which 40 differentially expressed primarily associated oxidation-reduction stress responses. More importantly, characterized miR477-CBP60A module response plant infection. A β-glucuronidase fusion reporter cleavage site analysis showed that ghr-miR477 directly cleaved messenger GhCBP60A posttranscriptional process. The ghr-miR477-silencing decreased resistance this fungus, while knockdown increased resistance, regulated GhICS1 expression determine salicylic acid level. Our data documented numerous later-inducible dahliae, suggesting these play important roles vascular disease.