Phosphorylation-Related Crosstalk Between Distant Regions of the Core Region of the Coat Protein Contributes to Virion Assembly of Plum Pox Virus

• DOI: 10.1094/mpmi-10-19-0305-r
• Publication Date: 2019-12-20T16:19:42Z
• Authors: Marta Hervás; Rosana Navajas; Mónica Chagoyen; Juan Antonio García; Sandra Martínez-T...
• Keywords: Crosstalk; Infectivity

Eukaryotic proteins are often targets of posttranslational modifications (PTMs). Capsid protein (CP) plum pox virus (PPV), a member genus Potyvirus, has been reported to be prone phosphorylation in four serines at the N-terminal region. CP proposed influence PPV infection by regulating accumulation coordination with second PTM, O-GlcNAcylation. In this study, further proteomic characterization revealed additional phospho-targets, thus evidencing even greater complexity network PTMs affecting protein. particular, two new targets, T254 and T313, distal core, appear highly relevant for infection. Although abolishing these positions does not have severe effect on infectivity or viral accumulation, phospho-mimicking either disrupts cell-to-cell movement. Strand-specific reverse transcription-quantitative PCR analysis fractionation centrifugation continuous sucrose gradient enabled us conclude that such deleterious is related failures replication but consequence inaccurate virion assembly. The spontaneous compensatory mutations core identified multiple mutant disclosed functional dialogue between distant which was supported an silico model, built watermelon mosaic atomic structure. Therefore, whereas joint opposite action O-GlcNAcylation disordered protrusion appears regulate stability, we propose phosphorylations region control assembly disassembly particles.