Parallel Genome-Wide Expression Profiling of Host and Pathogen During Soybean Cyst Nematode Infection of Soybean

• DOI: 10.1094/mpmi-20-3-0293
• Publication Date: 2007-05-11T09:42:59Z
• Authors: Nagabhushana Ithal; Justin Recknor; Dan Nettleton; Leonard Hearne; Tom Maier; Thomas J. Baum; Melissa G. Mitchum
• Keywords: Soybean cyst nematode; Nematode infection

Global analysis of gene expression changes in soybean (Glycine max) and Heterodera glycines (soybean cyst nematode [SCN]) during the course infection a compatible interaction was performed using Affymetrix GeneChip genome array. Among 35,611 transcripts monitored, we identified 429 genes that showed statistically significant differential between uninfected nematode-infected root tissues. These included encoding enzymes involved primary metabolism; biosynthesis phenolic compounds, lignin, flavonoids; related to stress defense responses; cell wall modification; cellular signaling; transcriptional regulation. 7,431 SCN 1,850 across different stages parasitism development. Differentially expressed were grouped into nine clusters based on their profiles roots. The patterns observed suggest coordinated regulation parasitism. Quantitative real-time reverse-transcription polymerase chain reaction confirmed results our microarray analysis. simultaneous genome-wide host pathogen provides new insights responses first profile transcript abundance occurring as it infects establishes permanent feeding site within plant root.