Characterization and Antifungal Properties of Wheat Nonspecific Lipid Transfer Proteins

• DOI: 10.1094/mpmi-21-3-0346
• Publication Date: 2008-02-07T21:39:22Z
• Authors: Jin-Yue Sun; Denis A. Gaudet; Zhen-Xiang Lu; Michele Frick; Byron Puchalski; André Laroche
• Keywords: Characterization

This study simultaneously considered the phylogeny, fatty acid binding ability, and fungal toxicity of a large number monocot nonspecific lipid transfer proteins (ns-LTP). Nine novel full-length wheat ns-LTP1 clones, all possessing coding sequences 348 bp, isolated from abiotic- biotic-stressed cDNA libraries aerial tissues, exhibited highly conserved regions with 78 to 99 71 100% identity at nucleotide amino levels, respectively. Phylogenetic analyses revealed two major ns-LTP families in wheat. Eight genes different clades were cloned into expression vector pPICZalpha transformed Pichia pastoris. Sodium dodecyl sulfate polyacrylamide gel electrophoresis, Western blotting, vitro activity assay confirmed that eight successfully expressed capable molecules. A comparative on mycelium growth or spore germination pathogens three nonwheat differential toxicities among ns-LTP. Values indicating 50% inhibition selected against six fungi ranged 1 7 microM. In lipid-binding was not correlated their antifungal activity. Using fluorescent probe SYTOX Green as an indicator membrane integrity, associated alteration permeability membranes.