Identification of R-Gene Homologous DNA Fragments Genetically Linked to Disease Resistance Loci in Arabidopsis thaliana

• DOI: 10.1094/mpmi.1998.11.4.251
• Publication Date: 2007-05-11T08:59:08Z
• Authors: Mark G. M. Aarts; Bas te Lintel Hek...; Eric B. Holub; Jim L. Beynon; Willem J. Stiekema; Andy Pereira
• Keywords: R gene

Disease resistance in plants is a desirable economic trait. A number of disease genes from various plant species have been cloned so far. The gene products some these can be distinguished by the presence an N-terminal nucleotide binding site and C-terminal stretch leucine-rich repeats. Although are structurally related, DNA sequences poorly conserved. Only parts share enough identity to design primers for polymerase chain reaction amplification related sequences. Such were used amplify different resistance-gene-like (RGL) fragments Arabidopsis thaliana accessions Landsberg erecta Columbia. Almost all genetically closely linked with known loci. Most RGL found clustered or dispersed multi-copy sequence organization, supporting supposed correlation