Four Classes of Salicylate-Induced Tobacco Genes

• DOI: 10.1094/mpmi.1998.11.9.895
• Publication Date: 2007-05-11T09:03:27Z
• Authors: Diana M. Horvath; Dorothy J. Huang; Nam-Hai Chua
• Keywords: Gene Expression Regulation, Viral; Nicotiana; 0301 basic medicine; DNA, Complementary; Molecular Sequence Data; Botany; hydrogen peroxide; Genes, Plant; Microbiology; QR1-502; Salicylates; 3. Good health; Tobacco Mosaic Virus; Kinetics; Plants, Toxic; 03 medical and health sciences; Gene Expression Regulation, Plant; QK1-989; differential display; Cloning, Molecular; Cycloheximide; auxin

We have identified and characterized fragments of 15 salicylic acid (SA) early response genes. The kinetics induction to cycloheximide (CHX) treatment allowed classification genes into four groups. Classes I-III are by immediate-early responses, showing increased accumulation mRNA within 30 min SA treatment. Moreover, CHX did not block these genes, indicating that latent cellular factors mediate the response. Class IV were induced more slowly, but still 2 3 h treatment, required protein synthesis for expression. Although in this study as SA-responsive several could also be other compounds. Two detail, including isolation cDNA sequences additional analysis gene Sequence revealed class I gene, C18-1, is previously ethylene element binding 1 (EREBP1), an ethylene-induced transcription factor basic pathogenesis-related (PR) whereas III G8-1, a novel sequence. G8-1 was found strongly only its active analogs exquisitely sensitive low concentrations. These activated at times following tobacco mosaic virus infection resistant genotypes.