A Profile of Putative Parasitism Genes Expressed in the Esophageal Gland Cells of the Root-knot Nematode Meloidogyne incognita

• DOI: 10.1094/mpmi.2003.16.5.376
• Publication Date: 2007-05-11T09:24:24Z
• Authors: Guozhong Huang; Bingli Gao; Tom Maier; R. Allen; Eric L. Davis; Thomas J. Baum; Richard S. Hussey
• Keywords: Root-knot nematode; Suppression subtractive hybridization

Identifying parasitism genes encoding proteins secreted from a nematode's esophageal gland cells and injected through its stylet into plant tissue is the key to understanding molecular basis of nematode plants. Meloidogyne incognita were cloned by microaspirating cytoplasm different parasitic stages provide mRNA create cell-specific cDNA library long-distance reverse-transcriptase polymerase chain reaction. Of 2,452 clones sequenced, deduced protein sequences 185 cDNAs had signal peptide for secretion and, thus, could have role in root-knot High-throughput situ hybridization with peptides resulted probes 37 unique specifically hybridizing transcripts accumulating within subventral (13 clones) or dorsal (24 M. incognita. In BLASTP analyses, 73% predicted novel proteins. Those similarities known included pectate lyase, acid phosphatase, hypothetical other organisms. Our analysis secretory provided, first time, profile putative expressed throughout cycle.