Grapevine red blotch virus: Absence in Swiss Vineyards and Analysis of Potential Detrimental Effect on Viticultural Performance

• DOI: 10.1094/pdis-07-17-1069-re
• Publication Date: 2017-11-06T13:31:11Z
• Authors: Jean-Sébastien Re...; Justine Brodard; Nathalie Dubuis; Vivian Zufferey; Olivier Schumpp; Santiago Schaerer; Paul Gugerli
• Keywords: Vineyard

Grapevine red blotch virus (GRBV) is a recently described that infects grapevine. Little information available on the possible occurrence and distribution outside North America. Therefore, we surveyed commercial vineyards from three major grape-growing regions in Switzerland to determine presence or absence of GRBV. In total, 3,062 vines were analyzed by polymerase chain reaction. None tested positive for GRBV, suggesting GRBV Swiss vineyards. We also investigated whether was present 653 grapevine accessions Agroscope collection at Nyon, including dominantly (457) but international accessions. Only six referential infected all originating United States, whereas others 10 European 8 non-European origins negative. High-throughput sequencing analysis Zinfandel A2V13, since 1985, confirmed close similarity isolate Z_A2V13 American isolates according genomes deposited GenBank. Because A2V13 reference maintained grafted leafroll indicator Vitis vinifera ‘Gamay’, evaluated effect viticultural performance over 3-year period. Our results showed clear detrimental effects physiology (vine vigor, leaf chlorophyll content, gas exchange) fruit quality. These findings underscore importance implementation testing worldwide certification quarantine programs prevent dissemination this virus.