Detection of Four New Tomato Viruses in Serbia Using Post Hoc High-Throughput Sequencing Analysis of Samples From a Large-Scale Field Survey

• DOI: 10.1094/pdis-09-20-1915-re
• Publication Date: 2021-03-25T03:56:46Z
• Authors: Ana Vučurović; Denis Kutnjak; Nataša Mehle; Ivana Stanković; Anja Pecman; Aleksandra Bulajić; Branka Krstić; Maja Ravnikar
• Keywords: virusi, patologija rastlin, paradižnik, odkrivanje, identifikacija; 0303 health sciences; virusi; viruses, tomato, detection, identification; detection; High-Throughput Nucleotide Sequencing; paradižnik; info:eu-repo/classification/udc/581.2; tomato; info:eu-repo/classification/udc/581; Plant Viruses; 3. Good health; patologija rastlin; 03 medical and health sciences; Solanum lycopersicum; odkrivanje; identifikacija; identification; viruses; Serbia; Plant Diseases

Tomato production worldwide is affected by numerous plant virus species. The early and accurate detection of viruses a critical step for disease control. However, the simultaneous most known tomato can be difficult because high number diversity tomato-infecting viruses. Here, we have identified four new in Serbia applying target-independent small RNA high-throughput sequencing (HTS). HTS was applied on pools samples separate samples, total comprising 30 that exhibited (severe) virus-like symptoms were collected during three annual surveys (2011 to 2013). These had previously tested negative presence 16 using targeted methods. Three divergent complete genome sequences Physostegia chlorotic mottled obtained from different localities, indicating first time this widespread might represent an emergent viral pathogen tomato. torrado detected at one locality with devastating yield losses. southern two spinach latent locality. In addition, already-known Serbia, spotted wilt orthotospovirus. All results subsequently confirmed study, successful application post hoc testing limited pooled resulted discovery Thus, our encourage use research diagnostic laboratories, including laboratories resources resolve etiology. [Formula: see text] Copyright © 2021 Author(s). This open access article distributed under CC BY-NC-ND 4.0 International license .