Development of a Molecular Tool for Identification of a NewNeopestalotiopsissp. Associated with Disease Outbreaks on Strawberry

• DOI: 10.1094/pdis-09-22-2117-re
• Publication Date: 2022-11-16T21:36:54Z
• Authors: Harleen Kaur; Jhulia Gelain; Marcus Vinicius M...; Natalia A. Peres; Guido Schnabel
• Keywords: Fragaria

A new Neopestalotiopsis sp. was recently reported causing outbreaks of leaf spot and fruit rot on strawberry in Florida, Georgia, South Carolina. In contrast to other Pestalotiopsis pathogens, the species appears more aggressive destructive strawberry. Current chemical options for management are disease suppressive at best, affected growers have been experiencing major yield losses. this study, we developed a molecular method based polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) identification from Isolates collected Florida; isolates N. rosae, honoluluana, ellipsopora, saprophytica, samarangensis, P. rhododendri; Carolina suspected be were included study. This is PCR amplification β-tubulin gene using previously published set primers (Bt2a Bt2b), followed by use restriction enzyme BsaWI. The cuts product twice, yielding fragments 290 base pairs (bp) 130 20 bp size, whereas only cut once, 420 bp. will aid research labs diagnostic clinics accurate fast variant