Resistance of Transgenic Prunus domestica to Plum Pox Virus Infection

• DOI: 10.1094/pdis.1997.81.11.1231
• Publication Date: 2007-05-11T09:46:47Z
• Authors: M. Ravelonandro; R. Scorza; J. C. Bachelier; G. Labonne; L. Levy; V. Damsteegt; A. M. Callahan; J. Dunez
• Keywords: clone (Java method); Potyviridae

Transgenic plum trees (Prunus domestica) containing the pox potyvirus coat protein (PPV-CP) gene were inoculated with PPV by aphid feeding or chip budding. Infection was monitored evaluation of virus symptoms, DAS-ELISA, and immunoblot assays. Based on observations analyses over 3 years including two dormancy cycles, one out five transgenic clones (C-5), found to be resistant infection whether aphids could not detected in any plants C-5 clone immunocap-ture-reverse transcriptase-polymerase chain reaction To our knowledge, this is first P. domestica produced genetic engineering.