First Report of Chickpea Chlorotic Dwarf Virus Infecting Spring Chickpea in Syria

• DOI: 10.1094/pdis.2004.88.4.424c
• Publication Date: 2007-05-11T10:24:52Z
• Authors: S. G. Kumari; K. M. Makkouk; N. Attar; W. Ghulam; D.-E. Lesemann
• Keywords: Polyclonal antibodies; Luteovirus

During May 2003, a high incidence of symptoms suggestive virus infection in spring chickpea were observed many fields Al-Ghab Valley, Syria, the ICARDA farm (near Aleppo, Syria), as well other locations northern including Idleb governorate. Symptoms yellowing, stunting, and necrosis. A total 1,345 samples with these (331 from 269 farm, 745 governorate) collected tested for presence five viruses tissue-blot immunoassay (TBIA) (4) at Virology Laboratory ICARDA, using following antisera: monoclonal antibodies Faba bean necrotic yellows (FBNYV, genus Nanovirus) (1); Bean leafroll (BLRV, family Luteoviridae) (4B10) (3); Beet western (BWYV, Polerovirus, Luteoviridae [ATCC PVAS-647, American Type Culture Collection, Manassas, VA]); Soybean dwarf (SbDV, Luteoviridae, PVAS-650]) polyclonal Chickpea chlorotic (CpCDV, Mastrevirus, Geminiviridae, provided by H. J. Vetten, BBA, Braunschweig, Germany). The most common present was BWYV (detected 54.1% tested), followed CpCDV (19.2%), BLRV (10.2%), FBNYV (5.5%). SbDV not detected any tested. Using immunosorbent electron microscopy, infected revealed low numbers geminivirus-like particles after 15 min incubation on antiserum-coated grids. When purified plants, coat protein 32 kDa sodium dodecyl sulfate-polyacrylamide gel electrophoresis typical (2) reacted strongly antiserum blots. vector Syria found to be Orosius albicinctus Distant, is thought similar orientalis (Matsumura), reported (2). FBNYV, BWYV, have been previously but our knowledge, this first report infecting Syria. References: (1) A. Franz et al. Ann. Appl. Biol. 128:255, 1996. N. M. Horn 122:467, 1993. (3) L. Katul. Characterization serology molecular biology leaf roll faba virus. Ph.D. thesis. University Gottingen, Germany, 1992. K. Makkouk Comeau. Eur. Plant Pathol. 100:71, 1994.