Identification and Functional Analysis of Tomato MicroRNAs in the Biocontrol Bacterium Pseudomonas putida Induced Plant Resistance to Meloidogyne incognita

• DOI: 10.1094/phyto-03-21-0101-r
• Publication Date: 2022-06-07T03:09:29Z
• Authors: Fan Yang; Ling Ding; Dan Zhao; Haiyan Fan; Xiaofeng Zhu; Yuanyuan Wang; Xiaoyu Liu; Yuxi Duan; Lijie Chen
• Keywords: Pseudomonas putida; Root-knot nematode

Root-knot nematodes (RKNs, Meloidogyne spp.) seriously damage tomato production worldwide, and biocontrol bacteria can induce immunity to RKNs. Our previous studies have revealed that Pseudomonas putida strain Sneb821 trigger against M. incognita several long noncoding RNAs microRNAs (miRNAs) are involved in this process. However, the molecular functions of miRNAs immune responses remain unclear. In study, deep small RNA sequencing identified 78 differentially expressed plants inoculated with relative alone; 38 were upregulated, 40 downregulated. The expression levels six known five novel validated using RT-qPCR assays. These included Sly-miR482d-3p, Sly-miR156e-5p, Sly-miR319a, novel_miR_116, novel_miR_121, novel_miR_221, which downregulated, Sly-miR390a-3p, Sly-miR394-3p, Sly-miR396a-3p, novel_miR_215, novel_miR_83, upregulated treated incognita. addition, Sly-miR482d was functionally characterized through gene silencing overexpression its target NBS-LRR (Solyc05g009750.1) by challenging inoculation. number second-stage juveniles (J2) inside roots induced galls significantly decreased both Sly-miR482d-silenced Solyc05g009750.1 overexpressing plants, whereas activity superoxide dismutase, peroxidase, hydrogen peroxide content increased. results suggest could inhibit thus regulate infestation. This study provides insights into bacteria-mediated engages plant miRNAs.