Markers Linked to Wheat Stem Rust Resistance Gene Sr11 Effective to Puccinia graminis f. sp. tritici Race TKTTF

• DOI: 10.1094/phyto-04-16-0165-r
• Publication Date: 2016-06-30T12:33:54Z
• Authors: Jayaveeramuthu Ni...; Shiaoman Chao; Pablo Olivera; Ebrahiem M. Babiker; Bekele Abeyo; Zerihun Tadesse; Muhammad Imtiaz; Luther Talbert; Nancy K. Blake; Eduard Akhunov; Michael O. Pumphrey; Yue Jin; Matthew N. Rouse
• Keywords: Stem rust; Puccinia; Germ plasm

Wheat stem rust, caused by Puccinia graminis f. sp. tritici, can cause severe yield losses on susceptible wheat varieties and cultivars. Although rust be controlled the use of genetic resistance, population dynamics P. tritici frequently lead to defeat resistance genes. race TKTTF a epidemic in Ethiopia Ug99-resistant ‘Digalu’ 2013 2014. The gene Sr11 confers is present ‘Gabo 56’. We identified seven single-nucleotide polymorphism (SNP) markers linked from cross between Gabo 56 ‘Chinese Spring’ exploiting 90K Infinium iSelect Custom beadchip. Five SNP were validated ‘Berkut’/‘Scalavatis’ that segregated for Sr11, using KBioscience competitive allele-specific polymerase chain reaction (KASP) assays. Two markers, KASP_6BL_IWB10724 KASP_6BL_IWB72471, predictive among stocks, cultivars, breeding lines North America, Ethiopia, Pakistan. These utilized select detect presence uncharacterized germplasm.