Draft Genome Sequence Resources of Three Strains (TOS4, TOS5, and TOS14) of Xylella fastidiosa Infecting Different Host Plants in the Newly Discovered Outbreak in Tuscany, Italy

• DOI: 10.1094/phyto-04-19-0108-a
• Publication Date: 2019-07-22T13:14:06Z
• Authors: Annalisa Giampetr...; Giusy D’Attoma; Stefania Zicca; Raied Abou Kubaa; Domenico Rizzo; Donato Boscia; Pasquale Saldarelli; Maria Saponari
• Keywords: 0301 basic medicine; Plant Disease; Plant Science; Xylella; Disease Outbreaks; Research and Innovation action; ST87; 03 medical and health sciences; SDSN - Greece; Xylella Fastidiosa Active Containment Through a multidisciplinary-Oriented Research Strategy; European Commission; Multiplex; Phylogeny; Plant Diseases; Xylella fastidiosa; Disease Outbreak; EC; H2020; Bacteriology; sdsn-gr; projects; DNA; Sequence Analysis, DNA; St87; multiplex; Europe; Italy; bacteriology; Sequence Analysis; Agronomy and Crop Science

An outbreak of Xylella fastidiosa was discovered in late 2018 in northern Italy affecting several plant species. Multilocus sequence typing analyses detected the presence of strains clustering in X. fastidiosa subsp. multiplex and harboring a hitherto uncharacterized sequence type, ST87. Three cultured strains (TOS4, TOS5, and TOS14) were subjected to high-throughput sequencing and the draft genomes assembled. Phylogenetic analysis conclusively indicated that they belong to the subspecies multiplex. The genetic information generated for these newly discovered strains further supports the evidence that sequence types are associated with the emergence of X. fastidiosa in Europe, posing major challenges for predicting the main threatened European and Mediterranean crops and plant species.