Characterization of the Endophytic Bacillus subtilis KRS015 Strain for Its Biocontrol Efficacy Against Verticillium dahliae

• DOI: 10.1094/phyto-04-23-0142-r
• Publication Date: 2023-08-02T12:32:20Z
• Authors: Jian Song; Dan Wang; Dongfei Han; Dan-Dan Zhang; Ran Li; Zhi-Qiang Kong; Xiao-Feng Dai; Krishna V. Subbarao; Jie-Yin Chen
• Keywords: Verticillium dahliae; Verticillium wilt; Endophyte; Verticillium; Wilt disease; Gossypium

Endophytes play important roles in promoting plant growth and controlling diseases. Verticillium wilt is a vascular disease caused by dahliae, widely distributed soilborne pathogen that causes significant economic losses on cotton each year. In this study, an endophyte KRS015, isolated from the seed of wilt-resistant Gossypium hirsutum ‘Zhongzhimian No. 2’, was identified as Bacillus subtilis morphological, phylogenetic, physiological, biochemical analyses. The volatile organic compounds (VOCs) produced KRS015 or its cell-free fermentation extract had antagonistic effects various pathogenic fungi, including V. dahliae. reduced index colonization dahliae treated seedlings significantly; reduction rate ∼62%. also promoted growth, potentially mediated growth-related genes GhACL5 GhCPD-3. triggered hypersensitivity response, reactive oxygen species (ROS) expression resistance-related genes. VOCs inhibited germination conidia mycelial were development-related such VdHapX, VdMcm1, Vdpf, Vel1. These results suggest potential agent for cotton.