Analysis of Plant and Fungal Transcripts from Resistant and Susceptible Phenotypes of Leptospermum scoparium Challenged by Austropuccinia psidii

• DOI: 10.1094/phyto-04-24-0138-r
• Publication Date: 2024-06-14T17:05:46Z
• Authors: Rebekah A. Frampton; Louise S. Shuey; Charles C. David; Georgia M. Pringle; Falk Kalamorz; Geoff S. Pegg; David Chagné; Grant R. Smith
• Keywords: 0303 health sciences; 03 medical and health sciences

Austropuccinia psidii is the causal pathogen of myrtle rust disease Myrtaceae. To gain understanding initial infection process, gene expression in germinating A. urediniospores and Leptospermum scoparium-inoculated leaves were investigated via analyses RNA sequencing samples taken 24 48 h postinoculation (hpi). Principal component transformed transcript count data revealed differential between uninoculated L. scoparium control plants that correlated with three plant leaf resistance phenotypes (immunity, hypersensitive response, susceptibility). Gene immune resistant did not significantly change response to fungal inoculation, whereas susceptible showed genes challenge. A putative gene, jg24539.t1, was identified phenotype family. Expression this may be associated could important for further Differential found hpi, but there no significant differences different phenotypes. There a decrease abundance transcripts encoding effectors carbohydrate-active enzyme suggesting encoded proteins are during phase infection. These transcripts, or their translated proteins, potential targets impede early phases by wide-host-range obligate biotrophic basidiomycete.