Identification and Characterization of Rice Circular RNAs Responding to Xanthomonas oryzae pv. oryzae Invasion

• DOI: 10.1094/phyto-06-21-0235-r
• Publication Date: 2021-08-23T04:51:29Z
• Authors: Peihong Wang; Sai Wang; Yan Wu; Wenhan Nie; Ayizekeranmu Yiming; Jin Huang; Iftikhar Ahmad; Bo Zhu; Gongyou Chen
• Keywords: 2. Zero hunger; 0303 health sciences; 03 medical and health sciences; Xanthomonas; Bacterial Proteins; Oryza; RNA, Circular; Plant Diseases

Emerging roles of circular RNAs (circRNAs) in various biological processes have advanced our knowledge transcriptional and posttranscriptional gene regulation. To date, no research has been conducted to explore their the rice-Xanthomonas oryzae pv. interaction. Therefore, we identified 3,517 circRNAs from rice leaves infected with highly virulent X. strain PXO99A by using rRNA depleted RNA sequencing technique coupled CIRI2 CIRCexplorer2 pipeline. Characterization analyses showed that these were distributed across whole genome rice, most arose exons (85.13%), ranged 200 1,000 bp, a noncanonical GT/AG (including CT/AC equivalent) splicing signal. Functional annotation enrichment analysis host genes produced differentially expressed (DEcircRNAs) suggested might play an important role reprogramming responses invasion, mainly mediating photorespiration chloroplast, peroxisome, diterpenoid biosynthesis. Moreover, 31 DEcircRNAs predicted act as microRNA decoys rice. The expression profile four validated quantitative real-time PCR divergent primers, back-splicing sites seven verified Sanger sequencing. Collectively, results inferred potential functional regulation immunity provide novel clues about molecular mechanisms rice-PXO99A