Virulence and Molecular Diversity in Cochliobolus sativus

• DOI: 10.1094/phyto.2001.91.5.469
• Publication Date: 2007-05-11T11:12:08Z
• Authors: Shaobin Zhong; Brian J. Steffenson
• Keywords: Cochliobolus sativus

Spot blotch, caused by the fungal pathogen Cochliobolus sativus, is an important disease of barley in many production areas world. To assess genetic diversity this pathogen, a worldwide collection C. sativus isolates was evaluated for virulence on and DNA polymorphism. Three pathotypes (0, 1, 2) were identified among 22 tested study 36 characterized previously three differentials (ND5883, Bowman, NDB112) that differ their resistance to sativus. Pathotype 2, which exhibits high cv. only found North Dakota, whereas other two occurred regions Genetic 58 isolates, together with related pathogenic spp. (C. heterostrophus, carbonum, victoriae) analyzed using amplified fragment length polymorphism (AFLP) markers. A total 577 polymorphic AFLP markers recorded 70 four eight primer combinations. Cluster analysis revealed distinct groups corresponding different species, except one case where race 0 carbonum placed outgroup may belong species. In 95 detected pairs used, each isolate exhibited unique pattern. Allelic pathotype 2 group lower (0.10) than (0.23) 1 (0.15) groups, indicating have arisen more recently. did not reveal close correlation between although identified. This low suggests exchange through parasexual recombination population. Some collected from world clustered into same group, suggesting migration around these has occurred.