Identification and Molecular Mapping of a Gene in Wheat Conferring Resistance to Mycosphaerella graminicola

• DOI: 10.1094/phyto.2003.93.9.1158
• Publication Date: 2007-05-11T11:22:41Z
• Authors: Tika B. Adhikari; Joseph M. Anderson; Stephen B. Goodwin
• Keywords: Mycosphaerella graminicola; Centimorgan; Graminicola; Molecular marker; Marker-Assisted Selection

Septoria tritici leaf blotch (STB), caused by the ascomycete Mycosphaerella graminicola (anamorph tritici), is an economically important disease of wheat. Breeding for resistance to STB most effective means control this and can be facilitated through use molecular markers. However, markers linked genes are not yet available. This study was conducted test in parents a standard wheat mapping population map any identified. The consisted 130 F 10 recombinant-inbred lines (RILs) from cross between synthetic hexaploid W7984 cv. Opata 85. Genetic analysis indicated that single major gene controls M. population. putative now designated Stb8 mapped with respect amplified fragment length polymorphism (AFLP) microsatellite An AFLP marker, EcoRI-ACG/MseI-CAG5, repulsion at distance approximately 5.3 centimorgans (cM). Two flanking markers, Xgwm146 Xgwm577, were on long arm chromosome 7B distances 3.5 cM, respectively. identified have potential marker-assisted selection breeding programs pyramiding other