Biopriming of Infected Carrot Seed with an Antagonist, Clonostachys rosea, Selected for Control of Seedborne Alternaria spp.

• DOI: 10.1094/phyto.2004.94.6.551
• Publication Date: 2007-05-11T11:23:51Z
• Authors: Birgit Jensen; Inge M. B. Knudsen; Mette Madsen; Dan Funck Jensen
• Keywords: Alternaria solani

ABSTRACT An ecological approach was used to select fungal antagonists effective against the seedborne pathogens Alternaria dauci and A. radicina on carrot. Twenty-five 105 isolates originating from cereal carrot habitats were screened in planta, respectively. Irrespective of isolate origin, belonging Clonostachys rosea controlled pre- postemergence death caused by as effectively fungicide iprodione. Isolate IK726 C. biopriming a seed lot with 29% 11% (highly infected), 4% 7% (low infection). Seeds primed water alone (hydropriming) or addition (biopriming). The occurrence increased twofold fivefold, respectively, during 14 days hydropriming, irrespective initial infection level. On highly infected seed, reduced incidence <2.3% that <4.8% while level both <0.5% bioprimed low rate. In sand stand establishment tests, hydroprimed seeds had lower healthy seedling than nonprimed seeds, mainly due high degree death. contrast, resulted better seeds. multiplied fivefold eightfold, microscopic observations using transformed green fluorescent protein (GFP) reporter gene showed covered fine web sporulating mycelium rosea. positive effect remained after 5 months storage at 4 degrees C survived numbers these seed. this study, we demonstrated bio-priming biocontrol strain facilitates priming without risking adverse effects establishment.