An Antibody Against the SecA Membrane Protein of One Phytoplasma Reacts with Those of Phylogenetically Different Phytoplasmas

• DOI: 10.1094/phyto.2004.94.7.683
• Publication Date: 2007-05-11T11:23:58Z
• Authors: Wei Wei; Shigeyuki Kakizawa; Hee-Young Jung; Shiho Suzuki; Minoru Tanaka; Hisashi Nishigawa; Shin-ichi Miyata; Kenro Oshima; Masashi Ugaki; Tadaaki Hibi; Shigetou Namba
• Keywords: Phytoplasma; Aster yellows; Strain (injury)

ABSTRACT Antisera raised against phloem-limited phytoplasmas generally react only with the phytoplasma strain used to produce antigen. There is a need for an antiserum that reacts variety of phytoplasmas. Here, we show SecA membrane protein onion yellows phytoplasma, which belongs aster 16S-group, detected eight strains from four distinct 16S-groups (aster yellows, western X, rice yellow dwarf, and elm yellows). In immunoblots, approximately 96-kDa was in plants infected each Immunohistochemical staining thin sections prepared localized phloem tissues. This should be useful detection histopathological analysis wide range