Biological Control of Grapevine Crown Gall Disease, Caused by Allorhizobium vitis, Using Paraburkholderia phytofirmans PsJN

• DOI: 10.1094/phytofr-03-22-0018-r
• Publication Date: 2022-11-15T23:35:32Z
• Authors: Nguyen-Huu Trong; Jeanne Doré; Matthieu Gaucher; Cédric Jacquard; Nicolas Richet; Vincent Leclère; Essaïd Aït Barka; Marie Noëlle Brisset; Isabelle Kerzaon; Céline Lavire; Christophe Clément; Ludovic Vial; Lisa Sanchez
• Keywords: 0301 basic medicine; Agrobacterium/Allorhizobium vitis; Botany; opine; Plant culture; Paraburkholderia phytofirmans PsJN; 630; [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy; grapevine; SB1-1110; 03 medical and health sciences; Paraburkholderia phytofirmans PsJN.; QK1-989; [SDV.BV]Life Sciences [q-bio]/Vegetal Biology; biocontrol; Agrobacterium /Allorhizobium vitis; crown gall disease

Controlling crown gall in grapevine ( Vitis vinifera L.) caused by the pathogenic bacterium Allorhizobium vitis is a major challenge for global viticulture, as this pathogen highly persistent vineyards once infected. The bacteria can enter plant through open wounds during pruning and then systemically colonize plant. This study aimed at evaluating potential of Paraburkholderia phytofirmans PsJN, beneficial endophytic able to xylem grapevine, same ecological niche A. vitis, control disease. P. PsJN was root-inoculated on plantlets before infection S4 shoots. level planta, vitopine production, accumulation lignin tumors, symptoms were investigated prebacterized or not with PsJN. expression 28 genes involved defense mechanisms also simultaneously determined quantitative real-time PCR. Despite direct antibacterial effect against AvS4, has no significant impact incidence disease severity. However, leads stronger tumors significantly reduced population planta. [Formula: see text] Copyright © 2022 Author(s). an access article distributed under CC BY-NC-ND 4.0 International license .